Author Topic: Backyard scientist on tissue cultures test tube babies  (Read 3330 times)

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Offline LouisianaMojo

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Backyard scientist on tissue cultures test tube babies
« on: November 13, 2018, 07:51:47 PM »
Doctor Mojo here as we get caught up here going into the spring next year will be showing a little bit more on the backyard tissue culture. There are few low cost studies we will be doing involving different techniques to see how they work here in the hot and humid South were in with different plant forms figs being the number one study of course.

Offline milmofigs

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Re: Backyard scientist on tissue cultures test tube babies
« Reply #1 on: November 20, 2018, 08:02:43 PM »
Interesting project Mojo, I'm interested in working tissue culture this upcoming year once I've got more plant tissue to work with. I've only got 2 small figs in ground and a few potted right now so I'm gonna wait it out some more, specially since I want to get better genetics to work with as well.
Do you already know the formula you'll be using for the tissue culture experiments? I've got a few papers I've downloaded from the web when I was investigating around, maybe you'd like me to send you them?
Looking forward to your progress, I'd appreciate if you posted some details on your procedure as far as cleaning the tissue and medium used.
Best of luck
MM

Offline ZebrasOfOz

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Re: Backyard scientist on tissue cultures test tube babies
« Reply #2 on: July 21, 2019, 05:27:20 PM »
Guys and Gals,

Been working over with the biology department folks. Have information coming in the next few weeks on how this is performed with the proper chemicals and nutrients. We know the basics already but the important information is the smaller micro nutrients and what amounts they are needed.


Christopher

Offline Ms Figgy

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Re: Backyard scientist on tissue cultures test tube babies
« Reply #3 on: December 01, 2019, 11:06:43 PM »
Thanks to all you guys!  I am planning to work on or try medicinal herbs so this is all important stuff!!

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« Last Edit: December 01, 2019, 11:16:26 PM by Ms Figgy »
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Offline Jhawka10

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Re: Backyard scientist on tissue cultures test tube babies
« Reply #4 on: December 01, 2019, 11:13:26 PM »
I have heard that tissue cultures are sterilized and the new plant will not have things like FMV in the trees.  If this is true how are they sterilized without killing the tissue?
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Offline LouisianaMojo

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Re: Backyard scientist on tissue cultures test tube babies
« Reply #5 on: December 02, 2019, 12:48:51 AM »
Boy oh boy let me take a stab at her. We need to keep in mind that the FMV is a virus. If you look at photos with a fig tree that has been infected the ones that are really infected are deformed and curled up.

Today's science is really very very far advance what we are dealing with with a tissue culture is a single cell structure a copy of the mothers plant to make a perfect clone the DNA structure. Banana plants they used the core or the internal bulb and sterilize it and cut Wafers off it to make Slants for test tubes.

With trees like figs we use the leaves and growth bud where the new Leaf is going to push from off the stem. Now you probably notice when it comes to Fig identification we look at the leaves this is the blueprint the fingerprint of that variety sound familiar.

So you probably noticed that you have some good looking Leafs normal-looking leaves and then you have some deformed leaves on a fig tree that's been infected we would choose a growth Bud off a good-looking section.

The sample is then taken to a clean room where it is sterilized seven or eight times changing the sterilization solution every time then it is transferred into a growing tube with the cell multiplier. This is a formula that Mass produce cell generation and replicates a perfect clone of that cell structure. This process will produce hundreds of perfect clones.

We also use the same process when cloning mushroom cultures that have been infected with bacteria. When dealing with Mushrooms cultures they sometime get infected with airborne bacteria or the wild green spores. Which you'll see green or black spots on your perfectly white culture inside your petri dish. How we remedy this is by taking a piece or a slant of the white part of that culture and placing it on the underside of new Ager and make it work and  recolonize the top of the petri dish we repeat this process several times to purify that mushroom culture.

Offline Jhawka10

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Re: Backyard scientist on tissue cultures test tube babies
« Reply #6 on: December 02, 2019, 10:46:57 AM »
Wow! Thats pretty amazing!
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Offline cornbread

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Re: Backyard scientist on tissue cultures test tube babies
« Reply #7 on: December 02, 2019, 04:17:25 PM »
Sounds interesting.  Plant tissue used in tissue culture is surface sterilized.  I would.think because FMV is a virus residing within the plant cell it can't be removed with normal tissue culture sterilization techniques.  Surface sterilization would remove bacteria and fungus though.

During sterilization the object is to sterilize the plant material long enouph to kill surface contaiminents without killing the explant.  This is the tricky part.

I have had good luck using Plant Preservative Mixture (PPM) to help cut down on culture contamination.  This was not on figs culture.

Please keep us updated on your progress.



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Offline LouisianaMojo

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Re: Backyard scientist on tissue cultures test tube babies
« Reply #8 on: December 02, 2019, 05:07:23 PM »
Cornbread I want to quit shoot this one. Sterilization on the outside is to keep bacteria from contaminating your culture. Your culture is then transferred into a cleanroom much like using a air flow Hood to keep contaminants away from your work.

What we're doing is like a mushroom tissue culture we sterilize the outside of the mushroom we put it into our clean room then we cut it open and take tissue Slants or cells from the inside which we use in Petri dishes for multiplication.

We can also compare this with like cancer you have cells and have cancer and then you have good cells that don't carry cancer we are after good cells. Now you hit the nail on the head it is a virus one that is caused by deficiencies with proper care and nutrients the immune system of the plant can fight it off.

You may do another comparison like herpes is a virus and you can't be contracted unless it's active. So what Laboratories are actually doing with tissue cultures they are extracting good healthy cells they then Mass multiplying them into a culture. We then neutralize the cell multiplication and then introduce the culture to rooting hormones to start the growing of roots and then you start your test tube babies that actually start growing a trunk and leaves. You have to remember all cells can be identified.

This is why all your commercial banana Growers used tissue cultures to keep diseases and viruses out of their plantations

Offline cornbread

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Re: Backyard scientist on tissue cultures test tube babies
« Reply #9 on: December 02, 2019, 07:38:04 PM »
I guess it depends where a latent virus resides and what part of the plant needs to be used for the culture.  Are most fig cultures done from axillary shoots?

It would be great if Southern Figs Forum could maintain a list of fig related research,  publications etc..


 
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